Funct. Mater. 2020; 27 (2): 283-295.
Synthesis and properties of magnetosensitive nanocomposites and ferrofluids based on magnetite, gemcitabine and HER2 antibody
1O.Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17 General Naumov Str., 03164 Kyiv, Ukraine
2I.Franko Zhytomyr State University, 40 V.Berdychevska Str., 10008 Zhytomyr, Ukraine
3R.Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, 45 Vasylkivska Str., 03022 Kyiv, Ukraine
The processes of adsorption of gemcitabine (GEM) on a surface of nanosized single-domain magnetite (Fe3O4) and magnetic properties of Fe3O4@GEM nanocomposites (NC) have been investigated. Ferrofluids (FF) were synthesized based on magnetite and physiological solution (PS), stabilized with sodium oleate (Ol.Na) and polyethylene glycol (PEG), containing GEM (Fe3O4@GEM/Ol.Na/PEG+PS). The properties of FF were investigated, as well as cytotoxic/cytostatic activity with respect to HepG2 hepatocellular carcinoma (HCC) cells of human liver. It was revealed that the complex action of GEM and her2 antibody (AB) in composition of FF Fe3O4@GEM/Ol.Na/PEG+PS+HER2 onto HepG2 cells caused the synergistic effect and an increase in cytotoxic activity by ~ 18-20 %, compared to GEM in mono-use, with a considerable decrease in its concentration.
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